Wednesday, December 14, 2022

The Main Trouble of Humanity is Misunderstanding of the Principles of Biological Systems Regulation and their Evolutionary Development

Introduction

According to thermodynamics, to control a biological system, no matter what system it is - microbes, plants or animals (including human society), two conditions must be met.
a) It is necessary to study and understand the features of internal regulation of this system. And having understood that:
b) Create most favorable conditions for its self-development. Do not force the system to change in the direction desired by certain individual, but to promote self-development, creating conditions required for that.
Unfortunately, this principle is most often not taken into account and not observed by the human. After all, to do it correctly, one needs to think carefully, instead of destroying everything around and showing his coolness. And which is most important, prevent violation of the objective laws of nature, which sooner or later would end with environmental disasters. In this regard, the slogan “human is the ruler of nature” should be considered the most harmful and strongly propagated by the authority’s thesis. This opinion arose under the influence of certain achievements of the human in the creation of highly productive plant varieties [1]. We should note that humans immediately absolutize every achievement, even minor, but a new one. When formation of ATP under the influence of light was discovered, it was immediately declared that the main regulation mechanism is ATP deficiency. After discovery of the regulatory involvement of cell membranes, everything was explained by a change in membrane permeability. They discovered genetic codes and genetics became the main thing in all the regulations. During the Lysenko period in Russia, genetics was suppressed, but then it returned to normal. But now the role of genes is being absolutized. And their role as an executive mechanism for the accumulation of information has become dominant, and even more important than metabolism itself.
But humans’ actions change a lot without understanding the consequences. Selecting plants with large fruits, people did not see what was happening with a root system that was outside their field of vision [2]. As a result, all cultivated plants have a root system 2-3 times smaller than that of their evolutionary predecessors. The consequences of that were tragic. A small root system provided an increased yield only with sufficient moisture and a high concentration of minerals in the soil. This promoted hydroponics. In such conditions, the use of mineral fertilizers has intensified with a simultaneous increase of plants quantity per unit of sown area (hectare). There was even an idea of “PROGRAMMING THE HARVEST”. Its principle is as follows. If you want to get a high yield, add the appropriate amount of minerals to the soil. In Europe, where soil fertility is insufficient and humidity is high, the role of mineral fertilizers has especially increased. While teaching economics and ANALYSIS of economic activity to students in the 1960s, I proudly told the students that in Holland they apply 1 ton of mineral fertilizers per hectare, whiler we (in Russia) are only 60-100kg / ha. At that time, students were told about the small root system of cultivated plants, that the plant does not need a large root size, since we give it all the necessary resources (water and minerals). And then we get a high yield.

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Tuesday, December 6, 2022

Sepsis Due to Co-Infection with Human Papillomavirus and Acinetobacter Schindleri after Surgery for Uterine Fibroids

Case

A 33-year-old woman underwent a physical examination at the Department of Gynecology and was found to have uterine fibroids. She was admitted for laparoscopic removal of the uterine fibroids under general anesthesia. During the operation, there was a small amount of dark red ascites in the pelvic cavity, a part of the intestine was densely adhered to the right pelvic wall, and the uterus was enlarged to the size of 10 weeks gestation, with an uneven surface. There was a fibroid tumor measuring approximately 7 × 8 × 8 cm on the posterior wall of the uterus, which was hard with clear boundaries. Two vesicular clear-fluid cysts with a diameter of 1-1.5 cm were seen on each fallopian tube. Due to the large posterior wall myoma and difficulty in suturing, the patient was considered barren; hence, abdominal myomectomy and pelvic adhesiolysis were selected, followed by indwelling catheterization. On the first day after surgery, the patient felt cold and was shivering, with a temperature of 38.5°C and lower abdominal pain. There was no sign of infection in the abdominal incision. When the indwelling catheter was removed, there was no urethral inflammation observed and the patient was able to urinate without difficulty. Blood was immediately drawn for culture and biochemical tests, which revealed low levels of sodium, calcium, and magnesium, and abnormal coagulation function. The level of original calcitonin was normal. The blood culture was positive after 15.3 hours in a nutrient solution for gram-negative bacilli. A diagnosis of sepsis was made based on the clinical features and laboratory values. Gram staining revealed short Gram-negative bacilli (Figure 1A). The blood culture plate showed gray and white colonies with good growth (Figure 1B). The chocolate culture plate showed poor growth (Figure 1C), while the MacConkey plate did not show any organism growth. (Figure 1D).

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Wednesday, November 30, 2022

Does Seed Inoculation with PGPRs Affect Germination and Final Biomass of Flax Under Drought Stress Conditions?

Seed germination as a primary aspect of growth is sensitive to water deficit. The current experiments were carried out to test the effects of drought stress and plant growth promoting rhizobacteria (PGPR) inoculation on seed germination, seedling growth, and biomass production of flax. Initially, the efficacy of PGPR (control, Bacillus amyloliquefaciens, Bacillus sp. strain1, Bacillus sp. Strain2, Azotobacter chroococcum, Pseudomonas putida, and Azospirillum lipoferum) and drought stress (0, -0.75, -1.5, -2 and -2.5 bar of PEG-6000) were estimated on flax germination under laboratory conditions. Then, bacterial treatments for the pot experiment were selected based on the laboratory experiment results (individually and in combination). Drought stress levels in the pot experiment were included 50%, 75%, and 100% crop water requirement. Results showed that the seeds inoculated with PGPRs under drought conditions positively affected seed germination and seedling growth under laboratory conditions. On the other hand, in the pot, emergence properties, dry biomass production, and root-related traits of bacterial inoculated plants were also improved compared with controls. B. amyloliquefaciens, Bacillus sp. strain1, and A. chroococcum in laboratory and coapplication of mentioned rhizobacteria in the pot recorded pronounced impact on most of the traits. Moreover, bacterial inoculation is proved to be an effective technique to increase the performance, growth and final biomass production of plants under unfavorable conditions like drought stress.

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Thursday, November 24, 2022

Monday, November 21, 2022

Carbon Nanomaterial and Solid-Phase Extraction: an Effective Adsorbent and Impressive Method for Measuring Norfloxacin in Biological Samples

Abstract

In recent years, drug use is on the rise, causing environmental pollution. Therefore, drug control is a common practice in many laboratories. This project focuses on increasing the method for determining small amounts of norfloxacin in aqueous and biological samples. Solid-phase extraction of small amounts of norfloxacin in aqueous samples using carbon nanotubes and visible and ultraviolet spectrophotometric measurements are used in biological samples. These systems include two phases, the aqueous donor phase and the acceptor phase of conjugated carbon nanomaterial.

Aqueous phase extraction and norfloxacin adsorption were performed in two experimental steps. First, methanol acidic solvent was used and the adsorbed samples were submitted to Vis-UV spectrophotometry for further analysis. Extraction parameters in this method This cheap and simple method is compatible with most tool analysis methods. These parameters include extraction time, adsorption of organic solvent effect, adsorption time, shaking time, the volume of donor phases, and optimized surfactant effect, and analysis and measurement were performed under optimal conditions. Low consumption of organic solvents, elimination of the effect of previous experiments, short extraction time, low detection threshold, and high concentration coefficient are the advantages of the mentioned technique and also the concentration factor and detection threshold for Norfloxacin were 49 and 8.96. The linear amplitude and relative standard were 1.46%.

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Friday, November 18, 2022

Biostimulation of Diesel Polluted Soil using Poultry Droppings

 Abstract

Bio stimulation is a form of bioremediation that involves the optimization of soil conditions such as pH, moisture, oxygen etc. to improve pollutant degradation. It embodies the introduction/ addition of nutrients to aid microbial growth. The impact of diesel pollution on the physicochemical properties of soil and the effects of remediation using poultry droppings was investigated. Sample soil, polluted with diesel was simulated in a single bio-treatment unit. Before contamination, the soil sample was characterized after digestion (using 1:1 ratio of 0.25M hydrochloric acid and Nitric acid) to determine the intrinsic properties of the soil. For effective treatment, a 2:1 mixture (w/w) of the polluted soil and the substrate in addition to 25ml of nutrient agar was utilized. The physicochemical properties of soil and rate of pollutant degradation after remediation was monitored for a period of 8 weeks. The initial total heterotrophic bacterial (THB) present in the experimental soil at week 1, was 2.44×105 cfu/g. The total heterotrophic bacterial after bioremediation was 3.74×105 cfu/ g. Also, the total hydrocarbon content (THC) decreased from an initial value of 7.89 mg/kg at week 1 to 2.67mg/kg at week 8. These results demonstrate the effectiveness of poultry droppings as a potent remediation tool for diesel polluted soil.

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Monday, November 14, 2022

Genetics and Human Intelligence

 Abstract

High intelligence is a valuable human capital that is correlated with success in one’s life. Human intelligence is governed by genetics as well as environmental factors. Recent studies indicate that contribution of genetics to intelligence is greater as compared to environment. Based on the above facts, we have gone through literature available on this topic. In this review article, we have focused on the genetic factors which influence human intelligence. Intelligence, in terms of IQ, is a polygenic trait and counting of genes linked to intelligence thus continues. Heritability of human intelligence increases with age. Genes of intelligence can protect a person from certain disorders but increase the vulnerability towards other disorders. Nowadays, Gene Plaza and DNA Land can predict the genetic IQ of a person. Few studies so far have been done on human intelligence and its relation with genetics. Hence, intense research is required to explore the genetics of human intelligence.

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FGFR Gene Mutation and Pfeiffer Syndrome

Abstract Pfeiffer syndrome is a genetic disease caused by a defect in the FGFR-1 or FGFR-2 genes. This syndrome affects the skeleton, whet...