Preparation of Generated DNA (Streptomyces Griseus) Crown Cells (Artificial Cells) and Antibiotic Production in Its’ Co-Cultures with Yeast (Beer) by Shoshi Inooka in Biotechnology and Microbiology-Lupine Publishers.
There has been significant progress in the generation of
artificial cells since the first studies in the 1960s [1-4], yet to
date, artificial cells that can replicate autonomously have not
been reported. Recently, the approaches for generating fully
operational (self-replicating) artificial cells have been reported
[5-7]. On the mechanism on underlying the formation of artificialcells, it was demonstrated that the artificial cells were covered
with DNA, (named DNA crown cells,) [8] and were generated when
they were incubated with white-egg. Thus, DNA crown cells are
artificial cells which the outside of the membrane were covered
with DNA and could replicated within egg white. These DNA crown
cells were formed as follows: first, Sph-DNA mixtures aggregates
with adenosine-lipids. From aggregates, DNA crown cells were
constructed with lipids [9,10]. Thus, the mechanism on the
formation of DNA crown cells became clear. Un-limited DNA crown
cells could be theoretically prepared. and using the methods, the
several kinds of DNA crown cells have been synthesized [11-14].
The detail on DNA crown cells (discovery, biological preparation,
synthesis and the mechanism of the formation, and so on) have
been described [6,15-18]. On the other hand, it is not clear whether
such DNA crown cells can contribute in the applied fields, such asbiomedicine or bio-industry- Lupine Publishers.
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