Cell Proliferation from the Assembly of Synthetic DNA (E. Coli) Crown Cells with Stimulation by Monolaurin Twice

 Abstract

DNA crown cells (artificial cells), in which the outside of the membrane is covered with DNA, can be readily synthesized in vitro using sphingosine (Sph)-DNA-adenosine mixtures to produce synthetic DNA crown cells. As these DNA crown cells can proliferate within egg whites, many kinds of synthetic DNA crown cells and DNA crown cells can be prepared using DNA from a variety of donors. Though the methods for synthesis are well established, how synthetic DNA crown cells are able to proliferate in egg whites remain unclear. Previous studies have demonstrated that assemblies of synthetic DNA (E. coli) crown cells were formed in response to the inorganic substances contained in egg white, and that modified synthetic DNA crown cells were produced within such assemblies. Moreover, the cells in these assemblies were not released after the addition of monolaurin. Rather, the assembly formed crystal-like substances and the cells disappeared. In the present study, it was examined whether cells were regenerated with two monolaurin treatments. Here, it was described that cells were produced from the assembly of synthetic DNA (E. coli) crown cells which form crystal-like substances with stimulation by monolaurin twice. The cell proliferation experiments were carried out as follows:

a) Synthetic DNA (E. coli) crown cells were incubated for 18 hours at 37℃ and these cells formed assemblies.

b)Monolaurin was added to the assemblies and the mixtures were incubated for 36 hours at 37℃.

c) The assemblies then transformed into crystal-like substances.

d)Monolaurin was added to these reconstructed synthetic DNA (E. coli) crown cells and the mixtures were incubated for 20–40 minutes at 37℃, which is when cell proliferation was observed.

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Prevalence of Hypothyroidism in Ischemic Heart Disease

Abstract

Cardiovascular diseases affect younger population in developing countries like India and carry a higher mortality rate. Common risk factors responsible for myocardial infarction in India are dyslipidemia, smoking, hypertension, diabetes, abdominal obesity, physical inactivity, low fruits and vegetables intake, and psychosocial stress. Other risk factors account for lesser number of cases, one such risk factor being hypothyroidism. A retrospective study was done using hospital records who were admitted with the diagnosis of acute coronary syndrome during the period January 2021 to February 2022 Department of Cardiology , Bangabandhu Sheikh Mujib Medical University (BSMMU) Dhaka, Bangladesh. A total number of 326 patients were admitted with acute coronary syndrome, out of these patients 93 (28.53%) were screened with thyroid profile(male 30, female 63). Female patients were more likely to be screened with thyroid profile than male patients (p value <0.0001). 17.2% (16) of the patients had thyroid abnormality. Three female patients had subclinical hypothyroidism. Four male and nine female patients had ‘low T3 syndrome’. Of the 93 patients there were 7 deaths (7.5%). In the low T3 group the mortality was 38.5%. The mortality in the low T3 group was significantly higher and statistically significant (p value 0.0062), compared to euthyroid subjects. The study highlights the fact that low T3 syndrome in patients with acute coronary syndrome (NSTEMI and STEMI) acts has a poor prognosis factor. The study also highlights to the fact that, screening is less commonly utilized in patients with acute coronary syndrome.

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