Cell Proliferation from the Assembly of Synthetic DNA (E. Coli) Crown Cells with Stimulation by Monolaurin Twice

 Abstract

DNA crown cells (artificial cells), in which the outside of the membrane is covered with DNA, can be readily synthesized in vitro using sphingosine (Sph)-DNA-adenosine mixtures to produce synthetic DNA crown cells. As these DNA crown cells can proliferate within egg whites, many kinds of synthetic DNA crown cells and DNA crown cells can be prepared using DNA from a variety of donors. Though the methods for synthesis are well established, how synthetic DNA crown cells are able to proliferate in egg whites remain unclear. Previous studies have demonstrated that assemblies of synthetic DNA (E. coli) crown cells were formed in response to the inorganic substances contained in egg white, and that modified synthetic DNA crown cells were produced within such assemblies. Moreover, the cells in these assemblies were not released after the addition of monolaurin. Rather, the assembly formed crystal-like substances and the cells disappeared. In the present study, it was examined whether cells were regenerated with two monolaurin treatments. Here, it was described that cells were produced from the assembly of synthetic DNA (E. coli) crown cells which form crystal-like substances with stimulation by monolaurin twice. The cell proliferation experiments were carried out as follows:

a) Synthetic DNA (E. coli) crown cells were incubated for 18 hours at 37℃ and these cells formed assemblies.

b)Monolaurin was added to the assemblies and the mixtures were incubated for 36 hours at 37℃.

c) The assemblies then transformed into crystal-like substances.

d)Monolaurin was added to these reconstructed synthetic DNA (E. coli) crown cells and the mixtures were incubated for 20–40 minutes at 37℃, which is when cell proliferation was observed.

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